|
Status |
Public on Jun 02, 2009 |
Title |
working myocardium _ stage e10.5 _ biorep2 |
Sample type |
RNA |
|
|
Source name |
working myocardium, stage e10.5, biorep2
|
Organism |
Mus musculus |
Characteristics |
working myocardium stage: E10.5
|
Growth protocol |
normal development
|
Extracted molecule |
total RNA |
Extraction protocol |
For every sample, transgenic hearts were dissected, the GFP-positive tissues pooled, dissociated to obtain single cells, and purified using FACS. 5000 cells per sample were gated and used for RNA isolation (Macherey-Nagel, nucleospin II). RNA was subjected to 2 rounds of linear amplification and biotin labeling (Ambion). Within each group 6 labeled antisense RNA samples were hybridized to separate Illumina Mouse-Ref-6 v1.1 BeadChips.
|
Label |
biotin
|
Label protocol |
RNA was subjected to 2 rounds of linear amplification and biotin labeling (Ambion). Within each group 6 labeled antisense RNA samples were hybridized to separate Illumina Mouse-Ref-6 v1.1 BeadChips.
|
|
|
Hybridization protocol |
standard Illumina hybridization protocol
|
Scan protocol |
standard Illumina hybridization protocol
|
Description |
W2-1C_E10.5
|
Data processing |
Data were processed using the R/Limma package (Bioconductor). Data were normalized using VSN transformation.
|
|
|
Submission date |
Nov 14, 2008 |
Last update date |
May 28, 2009 |
Contact name |
Henk Buermans |
E-mail(s) |
h.buermans@lumc.nl
|
Phone |
+31715268558
|
Organization name |
Leiden University Medical Center
|
Department |
Human Genetics
|
Lab |
Leiden Genome Technology Center
|
Street address |
Albinusdreef 2
|
City |
Leiden |
State/province |
ZH |
ZIP/Postal code |
2300 RC |
Country |
Netherlands |
|
|
Platform ID |
GPL6105 |
Series (1) |
GSE13614 |
Expression profiling of the forming atrioventricular node using a novel Tbx3-based node-specific transgenic reporter |
|