|
| Status |
Public on Oct 24, 2018 |
| Title |
wt_male_mph_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila males exposed to methylphenidate
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole flies
Sex: male
agent: methylphenidate
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed according to the manufracturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix protocol (GeneChipTM Expression Analysis Technical Manual)
|
| |
|
| Hybridization protocol |
15 microgram cDNA were fragmented and loaded onto the Affymetrix probe array cartridge. Incubation, washing and staining procedures were performed in the Affymetrix Fluidics Station 450
|
| Scan protocol |
The probe arrays were scanned at 560 nm using a confocal laser-scanning microscope (Affymetrix Scanner 3000 7G; Affymetrix, Santa Clara, CA, USA).
|
| Description |
Gene expression of males exposed to methylphenidate
|
| Data processing |
The transcriptomic data were normalized using the Robust Multi-array Average (RMA) algorithm (Irizarry et al., 2003) as implemented in the Affy package for R (Gautier et al., 2004). Differentially expressed transcripts were identified using the R package limma (Ritchie et al., 2015), by fitting linear models and empirical Bayesian methods, following a paired t-test and adjustment for multiple testing by Benjamin-Hochberg as implemented in limma.
|
| |
|
| Submission date |
Oct 23, 2018 |
| Last update date |
Oct 24, 2018 |
| Contact name |
Palle Duun Rohde |
| E-mail(s) |
palle.d.rohde@mbg.au.dk
|
| Organization name |
Aarhus University
|
| Department |
Department of Molecular Biology and Genetics
|
| Lab |
Center for Quantitative Genetics and Genomics
|
| Street address |
Blichers Alle 20
|
| City |
Tjele |
| ZIP/Postal code |
8830 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE121643 |
The genetic signature of variability in drug response in Drosophila melanogaster |
|
