NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM344190 Query DataSets for GSM344190
Status Public on Jan 15, 2009
Title BY4741 cells at T20, replicate #2
Sample type RNA
 
Source name S. cerevisiae cells 20 min after heat shock
Organism Saccharomyces cerevisiae
Characteristics BY4741
Treatment protocol Half of the cells were collected at T0 and half of the cells were subjected to 25 ºC-->37 ºC heat shock and cells were collected after 20 min, as samples T20.
Growth protocol S. cerevisiae cells were grown @ 25 ºC to O.D. 600 of 0.3~04, in triplicate.
Extracted molecule total RNA
Extraction protocol RiboPure kit were used to extract total RNA. Total RNAs were further purified using Qiagen RNAeasy Mini kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 300.
Description n/a
Data processing MAS 5
 
Submission date Nov 20, 2008
Last update date Jan 15, 2009
Contact name Cong Zhu
E-mail(s) cong.zhu@umassmed.edu
Phone 508-856-3756
Organization name UMass Medical School
Department Program in Gene Function and Expression
Street address 364 Plantation Street
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
 
Platform ID GPL2529
Series (1)
GSE13684 High-Resolution DNA Binding Specificity Analysis of Yeast Transcription Factors

Data table header descriptions
ID_REF
VALUE MAS 5 signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 172.0097
AFFX-BioB-M_at 256.3936
AFFX-BioB-3_at 178.3059
AFFX-BioC-5_at 337.1859
AFFX-BioC-3_at 561.7709
AFFX-BioDn-5_at 815.4237
AFFX-BioDn-3_at 2186.367
AFFX-CreX-5_at 5901.345
AFFX-CreX-3_at 7120.464
AFFX-DapX-5_at 392.2451
AFFX-DapX-M_at 718.2986
AFFX-DapX-3_at 672.9592
AFFX-LysX-5_at 21.5151
AFFX-LysX-M_at 31.74644
AFFX-LysX-3_at 123.0796
AFFX-PheX-5_at 53.43742
AFFX-PheX-M_at 68.50646
AFFX-PheX-3_at 83.81619
AFFX-ThrX-5_at 101.2933
AFFX-ThrX-M_at 123.9174

Total number of rows: 10928

Table truncated, full table size 216 Kbytes.




Supplementary file Size Download File type/resource
GSM344190.CEL.gz 838.4 Kb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap