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Sample GSM344223 Query DataSets for GSM344223
Status Public on Nov 21, 2008
Title EF2505 2
Sample type RNA
 
Source name EF2505-III treated
Organism Mus musculus
Characteristics 2h treatment of MLE-15 cells
Treatment protocol MLE-15 cells were treated with sham (PBS), NTHi lysate (100 ug/ml) or EF2505-III (40 ug/ml) for 2 hours.
Growth protocol MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
Extracted molecule total RNA
Extraction protocol total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description EF2505-III treated
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
 
Submission date Nov 20, 2008
Last update date Nov 20, 2008
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Unit 1462
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6105
Series (1)
GSE13685 Gene expression in respiratory epithelial cells treated to induce StIR

Data table header descriptions
ID_REF
VALUE Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_2417611 87.21639 46 4.405588 0.7398627
ILMN_1238136 108.6899 41 7.361815 0.209607
ILMN_2721178 197.7867 34 8.266964 0.01185278
ILMN_2458840 96.16172 37 4.398655 0.4697442
ILMN_1230777 17956.96 39 420.8546 0
ILMN_2537239 10088.33 39 296.3445 0
ILMN_1246069 11431.89 32 353.734 0
ILMN_2524361 144.7163 48 6.243293 0.03181535
ILMN_1243193 5801.877 34 160.7014 0
ILMN_1232042 89.55045 48 4.101787 0.6699938
ILMN_1242440 101.8658 51 4.163721 0.3275109
ILMN_2543689 109.5241 52 7.432209 0.1996257
ILMN_1233188 92.90053 31 5.637963 0.580786
ILMN_1224596 1395.181 40 38.13421 0
ILMN_1233643 120.9795 41 7.517621 0.106675
ILMN_1222139 651.0327 45 17.82544 0
ILMN_1259789 288.0966 44 12.13609 0.003742982
ILMN_2773981 2533.342 34 105.8234 0
ILMN_2771349 3260.589 41 125.749 0
ILMN_2634566 4415.835 46 158.9178 0

Total number of rows: 46632

Table truncated, full table size 1937 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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