|
| Status |
Public on Jan 28, 2019 |
| Title |
NHK: Normal keratinocytes RNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
keratinocyte from normal human skin tissue
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Normal keratinocytes
|
| Growth protocol |
Normal keratinocytes were cultured in Defined Keratinocyte Serum Free Medium (DKSFM, Life Technologies).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using RNeasy Plus (Qiagen) from the samples reported in this research. Ribosomal RNA was removed from each RNA extraction using Ribo-Zero Gold rRNA Removal kit (Illumina).
The RNA-seq libraries were constructed by TruSeq Stranded mRNA Library Prep kit (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Sequencing reads were mapped to hg19 using TopHat. FPKM values were called using cufflinks.
Genome_build: hg19
|
| |
|
| Submission date |
Oct 23, 2018 |
| Last update date |
Jan 28, 2019 |
| Contact name |
Anthony Oro |
| E-mail(s) |
oro@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Dermatology
|
| Lab |
Oro
|
| Street address |
269 Campus Drive, CCSR 2145c
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (2) |
| GSE108248 |
Epigenome regulation during epidermal lineage commitment [ATAC-seq, RNA-seq] |
| GSE122385 |
Epigenome regulation during epidermal lineage commitment |
|
| Relations |
| BioSample |
SAMN10282702 |
| SRA |
SRX4925288 |
