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| Status |
Public on Dec 11, 2018 |
| Title |
2PAE-2 |
| Sample type |
SRA |
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| Source name |
fat body
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| Organism |
Locusta migratoria |
| Characteristics |
Sex: Female
time point: 2
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| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using TRIzol
RNA library were prepared using standard Illumina protocols
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| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2500 |
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| Description |
miRNA_exp.xlsx
2P-3
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| Data processing |
Illumina Casava1.7 software used for basecalling.
The clean reads were obtained by eliminating the low-quality reads, empty adapters, reads shorter than 18 nt. Sequences of tRNA, rRNA, snRNA, snoRNA and piRNA were removed by similarity search using the Rfam12.1 and piRNABank database. miRNAs were identified by blasting the rest sequences with miRbase (V21.0) and referring to the locust genome
Then the RPKM value were calculated for expression level quantification.
Genome_build: V2.4.1
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| Submission date |
Nov 29, 2018 |
| Last update date |
Dec 11, 2018 |
| Contact name |
Jiasheng Song |
| E-mail(s) |
songj@vip.henu.edu.cn
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| Organization name |
Henan University
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| Street address |
Jinming RD
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| City |
Kaifeng |
| ZIP/Postal code |
475004 |
| Country |
China |
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| Platform ID |
GPL25868 |
| Series (1) |
| GSE123150 |
miRNA sequencing of fat body collect from adult female locusts |
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| Relations |
| BioSample |
SAMN10502033 |
| SRA |
SRX5081673 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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