|
| Status |
Public on Dec 10, 2009 |
| Title |
CD14+ at baseline, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
CD14+ monocytes, by positive selection
|
| Organism |
Homo sapiens |
| Characteristics |
female
|
| Treatment protocol |
CD14+ monocytes were isolated by density centrifugation (LymphoPrep) and successive positive selection with magnetic beads (Miltenyi).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolation including an on-column DNA-digestion step was performed with RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from 2µg total RNA with MessageAmp™ II-Biotin Enhanced Kit (Ambion).
|
| |
|
| Hybridization protocol |
Human Genome U133+ 2.0 GeneChip® microarrays were hybridized for 16h at 45°C with 15µg fragmented, biotin-labeled cRNA. Arrays were washed and stained using the GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and the Fluidics Station 450 (FS450_0004 script) .
|
| Scan protocol |
GeneChips were scanned with Affymetrix GeneChip Scanner 3000.
|
| Description |
gene expression data from peripheral blood mononuclear cells (PBMC) isolated from (fasting) blood samples before and after a 2 wk supplementation of 150mg quercetin per day
|
| Data processing |
The data was analysed with Bioconductor software package for R using the simpleaffy package (2.16.0-1) and normalisation method was gcrma (slow) with the gcrma package (2.12.1).
|
| |
|
| Submission date |
Dec 10, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Frank Döring |
| E-mail(s) |
sek@molprev.uni-kiel.de
|
| Organization name |
Christian-Albrechts-University Kiel
|
| Department |
Human Nutrition and Food Science
|
| Street address |
Heinrich-Hecht-Platz 10
|
| City |
Kiel |
| ZIP/Postal code |
24118 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE13899 |
Quercetin supplementation and CD14+ monocyte gene expression |
|
| Relations |
| Reanalyzed by |
GSE49910 |
| Reanalyzed by |
GSE119087 |
