|
| Status |
Public on Dec 13, 2018 |
| Title |
JFE011: BM37 Ind |
| Sample type |
RNA |
| |
|
| Source name |
Engineered human adipose tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone marrow-derived human stromal/stem cells differentiated into adipocyte tissue
|
| Growth protocol |
Cells were differenciated or not into adipocytes according to the protocol described in the associated publication. Reconstructed tissues were analysed at day 35 of culture.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RNeasy Lipid tissue Mini Kit (Qiagen), followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Dec 12, 2018 |
| Last update date |
Dec 13, 2018 |
| Contact name |
Julie Fradette |
| E-mail(s) |
julie.fradette@fmed.ulaval.ca
|
| Organization name |
Université Laval
|
| Department |
Chirurgie
|
| Lab |
LOEX - Julie Fradette
|
| Street address |
1401 18e Rue
|
| City |
Québec |
| State/province |
Québec |
| ZIP/Postal code |
G1J 1Z4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6097 |
| Series (1) |
| GSE123685 |
Genome-wide analysis of human reconstructed adipose tissue, human reconstructed connective tissue and native fat |
|
