|
Status |
Public on Mar 08, 2010 |
Title |
caPI3KSham_1417 |
Sample type |
RNA |
|
|
Source name |
Ntg MI
|
Organism |
Mus musculus |
Characteristics |
strain C57BL/6 Left ventricle
|
Treatment protocol |
As specified above for 6 mouse models of increasing cardiac stress.
|
Extracted molecule |
total RNA |
Extraction protocol |
Snap frozen heart samples in liquid nitrogen. TRIzol extraction of total RNA. Quality and integrity of small RNA assayed using Agilent Bioanalyzer.
|
Label |
Cy3
|
Label protocol |
Refer to Agilent miRNA labeling protocol
|
|
|
Hybridization protocol |
Refer to Agilent miRNA hybridisation protocol
|
Scan protocol |
Feature extraction 10.1 used
|
Description |
Agilent one-colour 8X15K miRNA microarray
|
Data processing |
Data were processed using alogorithms specified by the Agilent Feature Extraction software. Subsequent analysis of data were carried out using both Partek v6 and GeneSpring v10.
|
|
|
Submission date |
Jan 02, 2009 |
Last update date |
Mar 04, 2010 |
Contact name |
Ruby CY Lin |
E-mail(s) |
rubyl@unsw.edu.au, ruby.lin@sydney.edu.au
|
Organization name |
The Westmead Institute for Medical Research
|
Street address |
176 Hawkesbury Road, Westmead
|
City |
Sydney |
State/province |
NSW |
ZIP/Postal code |
2145 |
Country |
Australia |
|
|
Platform ID |
GPL8824 |
Series (1) |
GSE14267 |
microRNA profiling of myocardial infarction on a PI3K setting in mouse models |
|