|
Status |
Public on Jan 29, 2019 |
Title |
2_H3K27ac_ChIPseq |
Sample type |
SRA |
|
|
Source name |
TetO-TFAP2C hESC differentiation at Day7 with Dox
|
Organism |
Homo sapiens |
Characteristics |
cell type: early epidemal differentiated human emryonic stem cell (hESC) genotype: TetO-TFAP2C treatment and stage: early differentiaiton with Dox for 7 days chip antibody: H3K27ac; Active Motif Cat #39133
|
Growth protocol |
In vitro differentiation of TetO-TFAP2C H9 hESCs: To study the function of TFAP2C in surface ectoderm differentiation, TetO-TFAP2C H9 hESCs were induced with 2 mg/ml Doxycycline(Dox) in E6 medium without RA/BMP4 for 7 days. The resulting cells were imaged and collected for subsequent analysis.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared following the NEBNext protocol (NEBNext ChIP-Seq Library Prep kit Cat #E6240S/L)
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
bowtie used for alignment. peaks called using MACS Genome_build: hg19 Supplementary_files_format_and_content: bedGraph
|
|
|
Submission date |
Jan 29, 2019 |
Last update date |
Feb 04, 2019 |
Contact name |
Anthony Oro |
E-mail(s) |
oro@stanford.edu
|
Organization name |
Stanford University
|
Department |
Dermatology
|
Lab |
Oro
|
Street address |
269 Campus Drive, CCSR 2145c
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
|
|
Platform ID |
GPL18573 |
Series (2) |
GSE122385 |
Epigenome regulation during epidermal lineage commitment |
GSE125857 |
Epigenome regulation during epidermal lineage commitment [ChIP-seq] |
|
Relations |
BioSample |
SAMN10841532 |
SRA |
SRX5307285 |