|
| Status |
Public on Nov 16, 2009 |
| Title |
ENDOMETRIAL CELLS ESTRADIOL+PROGESTERONE+MONOCYTE-CONDITIONED MEDIUM 3 |
| Sample type |
RNA |
| |
|
| Source name |
CULTURED HUMAN ENDOMETRIAL CELLS (T-HESC) FROM ATCC
|
| Organism |
Homo sapiens |
| Characteristics |
Telomerase immortalized human endometrial cell line (ATCC #CRL-4003), passage 9, treated with 17beta estradiol (10-8 M) plus medroxyprogesterone acetate (10-7 M) for 8 days. Cells were serum starved days 6-8. Cells were treated with monocyte-conditioned medium (from U-937 cells) for 24 hours beginning on day 7.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The cultured endometrial cells were rinsed and TRI reagent (MRC) was added. The cells were scraped into tubes and centrifuged in the presence of bromochloropropane and sodium acetate. The RNA-containing layer was removed and the RNA purified on an RNeasy extraction column (Qiagen). The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen). The purity and quantity was evaluated with the RNA 6000 NanoLabChip in an Agilent Bioanalyzer.
|
| Label |
Alexa-647
|
| Label protocol |
see CodeLink application notes and manuals
|
| |
|
| Hybridization protocol |
see CodeLink application notes and manuals
|
| Scan protocol |
CodeLink Bioarrays scanned on Agilent G2565 microarray scanner
|
| Description |
Cells were grown in DMEM/F12 medium supplemented with 1% ITS+, 1% penicillin/streptomycin, and 500 ng/mL puromycin. 2% fetal bovine serum was also added to the medium from days 1-6.
|
| Data processing |
global median normalization, CodeLink Expression Analysis 4.1.0.4163
|
| |
|
| Submission date |
Jan 13, 2009 |
| Last update date |
Nov 16, 2009 |
| Contact name |
Kathleen M Eyster |
| E-mail(s) |
Kathleen.Eyster@usd.edu
|
| Organization name |
University of South Dakota
|
| Department |
Basic Biomedical Sciences
|
| Street address |
414 E. Clark St.
|
| City |
Vermillion |
| State/province |
SD |
| ZIP/Postal code |
57069 |
| Country |
USA |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE14808 |
Two-way communication between endometrial stromal cells and monocytes |
|
