|
Status |
Public on Nov 16, 2009 |
Title |
MONOCYTES CONTROL 3 |
Sample type |
RNA |
|
|
Source name |
CULTURED MONOCYTE CELL LINE (U-937) FROM ATCC
|
Organism |
Homo sapiens |
Characteristics |
U-937 monocytes, passage 12, were grown in suspension in RPMI-1640 supplemented with 10% charcoal/dextran treated FBS and 1% penicillin/streptomycin until the cells covered 80% of the flask surface. The medium was replaced with nonconditioned control medium for 24 hours.
|
Extracted molecule |
total RNA |
Extraction protocol |
The cultured U-937 monocyte cells were rinsed and TRI reagent (MRC) was added. The cells were scraped into tubes and centrifuged in the presence of bromochloropropane and sodium acetate. The RNA-containing layer was removed and the RNA purified on an RNeasy extraction column (Qiagen). The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen). The purity and quantity was evaluated with the RNA 6000 NanoLabChip in an Agilent Bioanalyzer.
|
Label |
Alexa-647
|
Label protocol |
see CodeLink application notes and manuals
|
|
|
Hybridization protocol |
see CodeLink application notes and manuals
|
Scan protocol |
CodeLink Bioarrays scanned on Agilent G2565 microarray scanner
|
Description |
Cells were grown in RPMI1640 medium supplemented with 1% penicillin/streptomycin and 10% fetal bovine serum.
|
Data processing |
global median normalization, CodeLink Expression Analysis 4.1.0.4163
|
|
|
Submission date |
Jan 13, 2009 |
Last update date |
Nov 16, 2009 |
Contact name |
Kathleen M Eyster |
E-mail(s) |
Kathleen.Eyster@usd.edu
|
Organization name |
University of South Dakota
|
Department |
Basic Biomedical Sciences
|
Street address |
414 E. Clark St.
|
City |
Vermillion |
State/province |
SD |
ZIP/Postal code |
57069 |
Country |
USA |
|
|
Platform ID |
GPL2895 |
Series (1) |
GSE14808 |
Two-way communication between endometrial stromal cells and monocytes |
|