|
| Status |
Public on Nov 16, 2009 |
| Title |
MONOCYTES +THESC CONDITIONED MEDIUM 2 |
| Sample type |
RNA |
| |
|
| Source name |
CULTURED MONOCYTE CELL LINE (U-937) FROM ATCC
|
| Organism |
Homo sapiens |
| Characteristics |
U-937 monocytes, passage 9, were grown in suspension in RPMI-1640 supplemented with 10% charcoal/dextran treated FBS and 1% penicillin/streptomycin until the cells covered 80% of the flask surface. The medium was replaced with THESC-conditioned medium for 24 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The cultured U-937 monocyte cells were rinsed and TRI reagent (MRC) was added. The cells were scraped into tubes and centrifuged in the presence of bromochloropropane and sodium acetate. The RNA-containing layer was removed and the RNA purified on an RNeasy extraction column (Qiagen). The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen). The purity and quantity was evaluated with the RNA 6000 NanoLabChip in an Agilent Bioanalyzer.
|
| Label |
Alexa-647
|
| Label protocol |
see CodeLink application notes and manuals
|
| |
|
| Hybridization protocol |
see CodeLink application notes and manuals
|
| Scan protocol |
CodeLink Bioarrays scanned on Agilent G2565 microarray scanner
|
| Description |
Cells were grown in RPMI1640 medium supplemented with 1% penicillin/streptomycin and 10% fetal bovine serum.
|
| Data processing |
global median normalization, CodeLink Expression Analysis 4.1.0.4163
|
| |
|
| Submission date |
Jan 13, 2009 |
| Last update date |
Nov 16, 2009 |
| Contact name |
Kathleen M Eyster |
| E-mail(s) |
Kathleen.Eyster@usd.edu
|
| Organization name |
University of South Dakota
|
| Department |
Basic Biomedical Sciences
|
| Street address |
414 E. Clark St.
|
| City |
Vermillion |
| State/province |
SD |
| ZIP/Postal code |
57069 |
| Country |
USA |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE14808 |
Two-way communication between endometrial stromal cells and monocytes |
|
