|
| Status |
Public on Feb 04, 2009 |
| Title |
131-ISS |
| Sample type |
RNA |
| |
|
| Source name |
breast cancer biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
DCIS stroma, tumor grade:II
|
| Treatment protocol |
laser capture microdissected
|
| Growth protocol |
fresh frozen primary breast cancer biopsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Picopure RNA extraction kit (Arcturus BioScience, Inc.)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared after two-rounds of T7-based amplification using the RiboAmp kit (Arcturus BioScience)
|
| |
|
| Hybridization protocol |
Hybridization, washing and staining performed per manufacturer's instructions (Affymetrix)
|
| Scan protocol |
GeneChips were scannned using GeneChip Scanner 3000.
|
| Description |
breast cancer
|
| Data processing |
Expression measures were extracted using the RMA algorithm with default parameters for background subtraction and quantile normalization.
|
| |
|
| Submission date |
Jan 23, 2009 |
| Last update date |
Feb 04, 2009 |
| Contact name |
Xiao-Jun Ma |
| E-mail(s) |
xiao-jun.ma@biotheranostics.com
|
| Organization name |
bioTheranostics, Inc.
|
| Street address |
11025 Roselle St, Suite 200
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
CA, 92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL1352 |
| Series (1) |
| GSE14548 |
Gene Expression Profiling of Tumor Microenvironment during Breast Cancer Progression |
|
