|
Status |
Public on Feb 13, 2009 |
Title |
Adjacent Tissue 83 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Human placental RNA (reference)
|
Organism |
Homo sapiens |
Characteristics |
tissue: placenta
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from frozen tissue according to the manufacturer’s instructions (Qiagen), and quality of the total RNA was judged based on the ratio of 28S/18S RNA after agarose gel electrophoresis.
|
Label |
Cy3
|
Label protocol |
The probe preparation was performed using 3DNA array detection system according to the manufacturer’s protocol (Genisphere, PA) with 20mg of total RNA.
|
|
|
Channel 2 |
Source name |
Adjacent liver tissue 83N
|
Organism |
Homo sapiens |
Characteristics |
tissue: liver
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from frozen tissue according to the manufacturer’s instructions (Qiagen), and quality of the total RNA was judged based on the ratio of 28S/18S RNA after agarose gel electrophoresis.
|
Label |
Cy5
|
Label protocol |
The probe preparation was performed using 3DNA array detection system according to the manufacturer’s protocol (Genisphere, PA) with 20mg of total RNA.
|
|
|
|
Hybridization protocol |
The hybridization was performed using 3DNA array detection system according to the manufacturer’s protocol (Genisphere, PA).
|
Scan protocol |
The arrays were scanned by ScanArray scanner (Perkin-Elmer)
|
Description |
Pair matched to 83T
|
Data processing |
Background subtraction and LOWESS normalization of log2 ratio (Cy5/Cy3) using sma R package
|
|
|
Submission date |
Feb 12, 2009 |
Last update date |
Feb 12, 2009 |
Contact name |
Bu-Yeo Kim |
Organization name |
Korea Institute of Oriental Medicine
|
Department |
Herbal Medicine Research Division
|
Street address |
1672 Yuseongdae-ro, Yuseong-gu
|
City |
Daejeon |
ZIP/Postal code |
305-811 |
Country |
South Korea |
|
|
Platform ID |
GPL8177 |
Series (1) |
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