|
| Status |
Public on Feb 13, 2009 |
| Title |
HCC 15 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Human placental RNA (reference)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: placenta
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen tissue according to the manufacturer’s instructions (Qiagen), and quality of the total RNA was judged based on the ratio of 28S/18S RNA after agarose gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
The probe preparation was performed using 3DNA array detection system according to the manufacturer’s protocol (Genisphere, PA) with 20mg of total RNA.
|
| |
|
| Channel 2 |
| Source name |
HCC 15T
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: hepatocellular carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen tissue according to the manufacturer’s instructions (Qiagen), and quality of the total RNA was judged based on the ratio of 28S/18S RNA after agarose gel electrophoresis.
|
| Label |
Cy5
|
| Label protocol |
The probe preparation was performed using 3DNA array detection system according to the manufacturer’s protocol (Genisphere, PA) with 20mg of total RNA.
|
| |
|
| |
| Hybridization protocol |
The hybridization was performed using 3DNA array detection system according to the manufacturer’s protocol (Genisphere, PA).
|
| Scan protocol |
The arrays were scanned by ScanArray scanner (Perkin-Elmer)
|
| Description |
Pair matched to 15N
|
| Data processing |
Background subtraction and LOWESS normalization of log2 ratio (Cy5/Cy3) using sma R package
|
| |
|
| Submission date |
Feb 12, 2009 |
| Last update date |
Feb 12, 2009 |
| Contact name |
Bu-Yeo Kim |
| Organization name |
Korea Institute of Oriental Medicine
|
| Department |
Herbal Medicine Research Division
|
| Street address |
1672 Yuseongdae-ro, Yuseong-gu
|
| City |
Daejeon |
| ZIP/Postal code |
305-811 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL8177 |
| Series (1) |
|
