|
| Status |
Public on Jan 01, 2010 |
| Title |
Sample 31 - AML-like |
| Sample type |
RNA |
| |
|
| Source name |
stored and frozen Bone Marrow cells
|
| Organism |
Homo sapiens |
| Characteristics |
disease: JMML
tissue: Bone Marrow
|
| Treatment protocol |
stored, frozen Bone Marrow or Peripheral Blood cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA purification using selective binding columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450DX.
|
| Scan protocol |
GeneChips were scanned using the Scan GCS3000Dx.
|
| Description |
Gene expression data from juvenile myelomonocytic leukemia samples at diagnosis
|
| Data processing |
The data were normalized using R software [www.r-ptoject.org] with BioConductor package [www.bioconductor.org]
|
| |
|
| Submission date |
Feb 17, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Andrea Zangrando |
| E-mail(s) |
andrea.zangrando@unipd.it
|
| Organization name |
University of Padova
|
| Department |
Woman and Child's Health
|
| Lab |
Hemato-Oncology
|
| Street address |
Via Giustiniani, 3
|
| City |
Padova |
| ZIP/Postal code |
35138 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14858 |
Gene exprssion profile classification predicts clinical outcome in juvenile myelomonocytic leukemia |
|
| Relations |
| Reanalyzed by |
GSE119087 |
