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Sample GSM372465 Query DataSets for GSM372465
Status Public on May 05, 2009
Title Nonatopic subject 13, HDM stimulated CD4 T-cells
Sample type RNA
 
Source name PBMC-derived HDM-stimulated CD4 T-cells at 24 h
Organism Homo sapiens
Characteristics time point: 24 h
Treatment protocol PBMC were cultured in the presence or absence of HDM extract (CSL).
Growth protocol PBMC from cryopreserved stocks were cultured in AIM-V medium for 24 hours.
Extracted molecule total RNA
Extraction protocol At the termination of the cultures, CD4 T-cell were isolated by immunomagnetic separation (DyNAL), and total RNA was extracted by TRIzol (Invitrogen) followed by RNeasy (QIAgen).
Label biotin
Label protocol Total RNA was labeled employing the Whole Transcript (WT) Sense Target Labeling Assay according to the manufacturer’s instructions (Affymetrix).
 
Hybridization protocol The samples were hybridized to HuGene ST1.0 microarrays according to standardized protocols from Affymetrix.
Scan protocol The samples were scanned on a GeneChip® Scanner 3000 7G (Affymetrix).
Description PBMC were from cryopreserved stocks.
Data processing The microarray data were preprocessed in Expression Consol software (Affymetrix) employing the probe logarithmic intensity error algorithm (PLIER, parameters - PM-GCBG background subtraction, quantile normalization, iterPLIER summarization).
 
Submission date Feb 19, 2009
Last update date Sep 01, 2016
Contact name Anthony Bosco
E-mail(s) Anthony.Bosco@telethonkids.org.au
Organization name Telethon Kids Institute
Street address 100 Roberts Road
City Subiaco
State/province WA
ZIP/Postal code 6008
Country Australia
 
Platform ID GPL6244
Series (1)
GSE14908 Global expression profiling of CD4 T-cell responses to house dust mite allergens in human atopics and nonatopics.
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE Signal on the log2 scale calculated by Expression Consol software.

Data table
ID_REF VALUE
7896736 5.062665729
7896738 4.263452727
7896740 4.451843319
7896746 8.227418116
7896748 9.704512525
7896750 7.397847447
7896752 9.493435186
7896754 7.238905296
7896756 5.581450075
7896759 5.918288191
7896761 8.127472329
7896779 7.862889613
7896798 7.859053911
7896817 7.545759145
7896822 6.690208946
7896859 6.254612182
7896861 4.809864573
7896863 5.439779033
7896865 6.739689624
7896878 7.402797202

Total number of rows: 33297

Table truncated, full table size 646 Kbytes.




Supplementary file Size Download File type/resource
GSM372465.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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