|
| Status |
Public on Apr 19, 2019 |
| Title |
left tumor (LT) treated with isotype control (A) at 1 wk, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
left tumor treated with isotype at 1 wk
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6J
age: 8-10 weeks at time of organ harvest
tissue: tumor
|
| Treatment protocol |
tumor implant protocol: Mice were implanted intradermally with 5x10^5 B16 tumor cells on Day 0.
Mice were injected intratumorally with MPL and CD40 and injected intraperitoneally with PD1 between Day 7-9. Other mice were injected intraperitoneally with PD1 alone. Treatment was given 2x/week and then sacrificed 1 week after start of trx.
organ harvest protocol: Mice were sacrificed and the distant tumors were dissected and mechanically dissociated into single cell suspensions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
not provided
|
| Label |
biotin
|
| Label protocol |
Biotinylated sense-strand DNA targets are generated from 50ng of total RNA following standard protocol using the WT Plus Reagent kit (Applied Biosystems GeneChip WT Plus Reagent Kit Target Preparation Manual).
|
| |
|
| Hybridization protocol |
Following fragmentation and labeling, 3.5ug of fragmented and labeled ss-cDNA was hybridized for 16hr at 45C on Clariom S Mouse HT array plates on the GeneTitan MultiChannel Instrument.
|
| Scan protocol |
After hybridization was completed the washing, staining and scanning of the arrays was completed on the GeneTitan MultiChannel Instrument.
|
| Description |
A3-LT_1Wk
|
| Data processing |
Data was analyzed using the rma() function in R package oligo
|
| |
|
| Submission date |
Apr 18, 2019 |
| Last update date |
Apr 19, 2019 |
| Contact name |
Nathan Suek |
| E-mail(s) |
nathansuek@gmail.com
|
| Phone |
6468882323
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Lab |
Lab of Jedd Wolchok
|
| Street address |
417 E 68th St Rm Z1525
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL24242 |
| Series (1) |
| GSE130027 |
In situ vaccination with defined factors overcomes T cell exhaustion in distant tumors [expression data] |
|
