|
| Status |
Public on Feb 26, 2009 |
| Title |
43h mosquito embryo RNA, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
43h mosquito embryo
|
| Organism |
Anopheles gambiae |
| Characteristics |
strain: Kisumu
|
| Treatment protocol |
Staged mosquito eggs were collected into meshed basket, treated in 25% household bleach for 1min.16 seconds, washed thoroughly with streaming water and subjected to RNA prep.
|
| Growth protocol |
Mosquito eggs were aged in water at 25C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction were performed by Trizol, followed by purification on RNAeasy (Qiagen) column.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix one-step protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Regular, following the guidelines from Affymetrix.
|
| Scan protocol |
Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix).
|
| Description |
for further information please refer to (Goltsev et al, Developmental and evolutionary basis for drought tolerance of the Anopheles gambiae embryo. 2009, Developmental Biology)
|
| Data processing |
RMA-express software was used for processing of raw expression data and estimation of log base 2 expression values.
|
| |
|
| Submission date |
Feb 25, 2009 |
| Last update date |
Feb 25, 2009 |
| Contact name |
Yury Goltsev |
| E-mail(s) |
goltsev@berkeley.edu, mlevine@berkeley.edu
|
| Phone |
510 642 5007
|
| Organization name |
UC Berkeley
|
| Department |
MCB
|
| Lab |
Levine
|
| Street address |
585 LSA, UC Berkeley
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platform ID |
GPL1321 |
| Series (2) |
| GSE14993 |
Developmental time course of gene expression in Anopheles gambiae embryo |
| GSE15001 |
Gene expression in the Anopheles gambiae embryo |
|
