|
| Status |
Public on Apr 10, 2009 |
| Title |
Breast cancer cell line: BT20 (Batch 1) |
| Sample type |
RNA |
| |
|
| Source name |
Breast Cancer Cell Line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BT20
cell type: Breast Cancer
colony number: 1564.333333
batch: 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Snap-frozen samples in liquid nitrogen were used for extraction. Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from breast cancer cell line
0159_6153_h133+_BT20
|
| Data processing |
Data were normalized by RMA using R with Bioconductor. ComBat standardization was then used to remove the batch effect.
|
| |
|
| Submission date |
Feb 26, 2009 |
| Last update date |
Dec 17, 2018 |
| Contact name |
Seiichi Mori |
| E-mail(s) |
seiichi.mori@nus.edu.sg
|
| Phone |
(65) 6516 1148
|
| Fax |
(65) 6873 9664
|
| URL |
http://www.csi.nus.edu.sg
|
| Organization name |
National University of Singapore
|
| Department |
Cancer Science Institute Singapore
|
| Lab |
Genomic Oncology Programme
|
| Street address |
Centre for Life Sciences #02-07, 28 Medical Drive
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
117456 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE15026 |
Expression data from breast cancer cell lines with various colony-forming ability |
| GSE15181 |
Expression profiles of cancer cells with anchorage-independent growth ability |
|
| Relations |
| Reanalyzed by |
GSE123917 |
