|
| Status |
Public on Oct 16, 2019 |
| Title |
pH4_T2_2 |
| Sample type |
SRA |
| |
|
| Source name |
Liquid culture
|
| Organism |
Candida albicans |
| Characteristics |
ph: 4
time: 2 hours
replicate: 2
|
| Growth protocol |
Cells were grown in YPD buffered at pH4 or pH6 at 37c, 200 rpm.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy kit (Qiagen)
RNA libraries were produced using the Biomek FxP (Beckman Coulter A31842) with NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolab E7420L) and NEBnext Multiplex Oligos for Illumina Dual Index Primers (New England Biolabs E7600S)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
TPM summary.xlsx
|
| Data processing |
Trim reads, CLC Genomics Workbench 11.0.1
Reads alignement, CLC Genomics Workbench 11.0.1
Genome_build: A21-s02-m09-r10
Supplementary_files_format_and_content: TPM
|
| |
|
| Submission date |
May 09, 2019 |
| Last update date |
Oct 16, 2019 |
| Contact name |
Rebecca Hall |
| E-mail(s) |
R.A.Hall@bham.ac.uk
|
| Organization name |
University of Birmingham
|
| Department |
IMI
|
| Lab |
HAPI group
|
| Street address |
University of Birmingham, School of Biosciences
|
| City |
Birmingham |
| ZIP/Postal code |
B15 2TT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL24129 |
| Series (1) |
| GSE130948 |
Re-masking of fungal cell wall PAMPs in response to environmental pH is regulated by multiple processes |
|
| Relations |
| BioSample |
SAMN11609420 |
| SRA |
SRX5811024 |
