|
| Status |
Public on Jun 30, 2011 |
| Title |
Patient5_339_GH_Day3_GH2_2AM (4 days) |
| Sample type |
RNA |
| |
|
| Source name |
Milk Fat Globule
|
| Organism |
Homo sapiens |
| Characteristics |
sample: Milk Fat Globule
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
|
| Label |
Biotin
|
| Label protocol |
440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
|
| |
|
| Hybridization protocol |
750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
|
| Scan protocol |
Probe arrays were scanned using the BeadArray reader (Factor 1)
|
| Description |
Day3_GH2_2AM
|
| Data processing |
Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
|
| |
|
| Submission date |
Mar 12, 2009 |
| Last update date |
Jun 30, 2011 |
| Contact name |
Patricia Maningat |
| E-mail(s) |
mmaningat@mail.rockefeller.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
CNRC
|
| Street address |
1100 Bates St
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE15208 |
Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days |
|
