|
| Status |
Public on Jun 03, 2009 |
| Title |
Transgenic line 4, biological rep. 2 |
| Sample type |
RNA |
| |
|
| Source name |
15 days old rice shoot unstressed
|
| Organism |
Oryza sativa Indica Group |
| Characteristics |
cultivar: Pusa Basmati 1
tissue: 15 days old rice shoot
genotype/variation: transgenic
|
| Treatment protocol |
Untreated seedlings were used for shoot tissue
|
| Growth protocol |
Seedlings were raised on MS medium without antibiotic selection for 15 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 450C on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene Chip Scanner 3000
|
| Description |
Gene espression data of 15 days old rice shoot in codA expressing transgenic line4
|
| Data processing |
First-order analysis was performed using Affymetrix Gene Chip Operating (GCOS) software 1.2.1. Normalization of data and differential expression analysis was done using Avadis 4.2 prophetic software (Strandgenomics, Bangalore, India)
|
| |
|
| Submission date |
Mar 30, 2009 |
| Last update date |
Jun 03, 2009 |
| Contact name |
Akhilesh Kumar Tyagi |
| E-mail(s) |
akhilesh@genomeindia.org
|
| Phone |
91-11-24115095
|
| Fax |
91-11-24115095
|
| Organization name |
University of Delhi South Campus, Benito Juarez Road India
|
| Department |
Plant Molecular Biology
|
| Street address |
Benito Juarez Road
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110021 |
| Country |
India |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE15448 |
Glycinebetaine-induced water-stress tolerance in codA-expressing transgenic indica rice |
|
