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Status |
Public on Jun 03, 2009 |
Title |
Transgenic line 22, biological rep. 2 |
Sample type |
RNA |
|
|
Source name |
15 days old rice shoot unstressed
|
Organism |
Oryza sativa Indica Group |
Characteristics |
cultivar: Pusa Basmati 1 tissue: 15 days old rice shoot genotype/variation: transgenic
|
Treatment protocol |
Untreated seedlings were used for shoot tissue
|
Growth protocol |
Seedlings were raised on MS medium without antibiotic selection for 15 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA
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|
|
Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 450C on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
Scan protocol |
GeneChips were scanned using the Affymetrix Gene Chip Scanner 3000
|
Description |
Gene espression data of 15 days old rice shoot in codA expressing transgenic line22
|
Data processing |
First-order analysis was performed using Affymetrix Gene Chip Operating (GCOS) software 1.2.1. Normalization of data and differential expression analysis was done using Avadis 4.2 prophetic software (Strandgenomics, Bangalore, India)
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|
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Submission date |
Mar 30, 2009 |
Last update date |
Jun 03, 2009 |
Contact name |
Akhilesh Kumar Tyagi |
E-mail(s) |
akhilesh@genomeindia.org
|
Phone |
91-11-24115095
|
Fax |
91-11-24115095
|
Organization name |
University of Delhi South Campus, Benito Juarez Road India
|
Department |
Plant Molecular Biology
|
Street address |
Benito Juarez Road
|
City |
New Delhi |
State/province |
Delhi |
ZIP/Postal code |
110021 |
Country |
India |
|
|
Platform ID |
GPL2025 |
Series (1) |
GSE15448 |
Glycinebetaine-induced water-stress tolerance in codA-expressing transgenic indica rice |
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