|
Status |
Public on Aug 24, 2009 |
Title |
fenofibrate_treated_HUVEC_18hr-1 |
Sample type |
RNA |
|
|
Source name |
HUVEC
|
Organism |
Homo sapiens |
Characteristics |
tissue: HUVEC drug treatment time point: 18hr
|
Treatment protocol |
HUVEC pools consisting of 10 biological isolates were treated with 25umol fenofibrate (Sigma-Aldrich, UK) dissolved in DMSO (Sigma-Aldrich, UK). Cells were incubated at 37ºC/5%CO2 for the time points in transcriptional profiling.
|
Growth protocol |
HUVECs were isolated from umbilical cords by collagenase digestion and cultured at 37ºC/5%CO2 in basal culture medium supplemented with a proprietary mixture of heparin, hydrocortisone, epidermal growth factor, FGF and 2% foetal calf serum (FCS; EGM-2, Cambrex, Workingham, UK).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was prepared from fenofibrate-treated or -untreated HUVECs using Trizol reagent (Invitrogen)
|
Label |
biotin
|
Label protocol |
Biotin-labelled complex cRNAs were prepared to according to the manufacturer's protocols (Applied Microarrays, formally supplied by GE Healthcare)
|
|
|
Hybridization protocol |
Biotin-labelled complex cRNAs were hybridized to CodeLink UniSet I Human 20K according to the manufacturer's protocols (Applied Microarrays, formally supplied by GE Healthcare).
|
Scan protocol |
The arrays were scanned with Agilnet DNA Microarray Scanner model G2565BA.
|
Description |
none
|
Data processing |
The scanned images were quantified with CodeLink Expression Array Software (Applied Microarrays, formally supplied by GE Healthcare), where the intensity of each spot was divided by the spot’s signal median.
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|
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Submission date |
Mar 31, 2009 |
Last update date |
Aug 24, 2009 |
Contact name |
D. Stephen Charnock-Jones |
E-mail(s) |
dscj1@cam.ac.uk
|
Organization name |
University of Cambridge
|
Department |
Obstetrics and Gynaecology
|
Street address |
The Rosie Hospital
|
City |
Cambridge |
ZIP/Postal code |
CB2 0SW |
Country |
United Kingdom |
|
|
Platform ID |
GPL4044 |
Series (2) |
GSE15483 |
The effect of fenofibrate on endothelial cells |
GSE15494 |
Human umbilical vein endothelial cells (HUVECs) treated with fenofibrate |
|