|
| Status |
Public on Dec 31, 2020 |
| Title |
soil_CNT_30_rep2 |
| Sample type |
genomic |
| |
|
| Source name |
soil with 30 mg/kg carbon nanotubes
|
| Organism |
uncultured soil microorganism |
| Characteristics |
group: CNTs
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted from soil samples and then purified
|
| Label |
Cy5
|
| Label protocol |
after amplified, samples were labeled with the fluorescent dye Cy-5 using a random priming method
|
| |
|
| Hybridization protocol |
the labeled DNA was hybridized onto the GeoChip 5.0 microarray, then the arrays were washed before scanning
|
| Scan protocol |
the arrays were scanned by the NimblGen MS200 Microarry Scanner
|
| Description |
functional genes
|
| Data processing |
ImaGene 6.0 software was used to convert optical signals into digital ones and only those spots automatically scored positively in the raw data were used for downstream analysis. Spots with a signal-to noise ratio below 2.0 were removed. And the raw data were then normalized.
normalized intensity of each detected genes were list in the table.
|
| |
|
| Submission date |
Jul 31, 2019 |
| Last update date |
Dec 31, 2020 |
| Contact name |
fan wu |
| E-mail(s) |
wufanfan@pku.edu.cn
|
| Phone |
17710051371
|
| Organization name |
Peking university
|
| Street address |
NO.5 Yiheyuan Road Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
| |
|
| Platform ID |
GPL22263 |
| Series (1) |
| GSE135153 |
soil mcirobial functional communities |
|
