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| Status |
Public on Oct 14, 2020 |
| Title |
OKH,transcriptome 01 |
| Sample type |
SRA |
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| Source name |
OKH,transcriptome 01
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| Organism |
Didelphis virginiana |
| Characteristics |
tissue: Kidney Cells
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| Growth protocol |
Cell lines were grown on transwell (see manuscript) to confluence before RNA isolation.
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNAs were extracted using Zymo kit and cDNAs were sythesized using SMART-Seq V4 Ultra Low Input RNA Kit.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
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| Data processing |
Sequenced reads were mapped to Ensembl Release 94 genome using STAR-2.5.4a
Transcript abundances in TPM were caculated by rsem 1.3.0
Genome_build: Ensembl Release 94
Supplementary_files_format_and_content: tab-delimited text files include TPM values for each sample
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| Submission date |
Aug 06, 2019 |
| Last update date |
Oct 14, 2020 |
| Contact name |
Lihe Chen |
| E-mail(s) |
lihe.chen@nih.gov
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| Phone |
3015947152
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| Organization name |
NHLBI
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| Street address |
10 center drive MSC 1603, Building 10, Room 6N318
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platform ID |
GPL27016 |
| Series (1) |
| GSE135441 |
Transcriptomes of Major Proximal-Tubule Cell-Culture Models |
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| Relations |
| BioSample |
SAMN12501546 |
| SRA |
SRX6658772 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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