|
Status |
Public on Dec 08, 2021 |
Title |
rancRNA_18-M2_total mRNA_rep2 |
Sample type |
SRA |
|
|
Source name |
yeast strain trm10∆
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
strain: trm10<delta> electroporation: rancRNA_18-M2 mrna fraction: total
|
Extracted molecule |
total RNA |
Extraction protocol |
Yeast spheroplasts were electroporated with or without RNA, recovered and harvested by centrifugation. Pellets were opened using a FastPrep. Lysates were cleared by centrifugation and total RNA was extracted by hot acid phenol extraction. Poly-A-Enrichment via oligo-dT-beads. PolyA enriched mRNAs were transformed into a cDNA library using the Illumina TruSeq stranded mRNA Kit. Library size was determined using Qbit.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 3000 |
|
|
Description |
mSeq of poly-A enriched RNA mRNA
|
Data processing |
Quality control using fastqc tool. Mapping performed using HISAT2; more than 95% mapping rate, -p 8. Counting was performed using featureCounts. Genome_build: Strain BY4742 (PRJNA260311, Oct-12-2014) Supplementary_files_format_and_content: *.txt: Tab-delimited text file; counts table.
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|
|
Submission date |
Aug 06, 2019 |
Last update date |
Dec 08, 2021 |
Contact name |
Norbert Polacek |
E-mail(s) |
ag.polacek@gmail.com, norbert.polacek@dcb.unibe.ch
|
Organization name |
University of Bern
|
Department |
Chemistry and Biochemistry
|
Street address |
Freiestrasse 3
|
City |
Bern |
ZIP/Postal code |
3012 |
Country |
Switzerland |
|
|
Platform ID |
GPL23014 |
Series (1) |
GSE135475 |
mRNA seq of total and polysome associated mRNAs in three different conditions |
|
Relations |
BioSample |
SAMN12511312 |
SRA |
SRX6662824 |