|
Status |
Public on May 12, 2010 |
Title |
foxo4 knock out mice without treatment |
Sample type |
RNA |
|
|
Source name |
colon mucosa
|
Organism |
Mus musculus |
Characteristics |
strain: FVB/N6 age: 8 weeks genetic modification: foxo4 knock out tissue: Colon Mucosa treatment: untreated
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 5
|
Data processing |
The data were normalized using quantile normalization with IlluminaGUI in R
|
|
|
Submission date |
May 08, 2009 |
Last update date |
May 12, 2009 |
Contact name |
wen zhou |
E-mail(s) |
wen.zhou@utsouthwestern.edu
|
Phone |
214-648-4024
|
Organization name |
UT Southwestern Medical Center at Dallas
|
Department |
Internal Medicine ,Cardiology
|
Lab |
Liu Lab
|
Street address |
6000 Harry Hines Blvd NB11.110
|
City |
Dallas |
State/province |
TX |
ZIP/Postal code |
75235 |
Country |
USA |
|
|
Platform ID |
GPL6105 |
Series (1) |
GSE16033 |
Microarray analysis of colonic mucosa gene expression in wild type and foxo4 knock out mice |
|