|
| Status |
Public on Dec 10, 2019 |
| Title |
Relapse exercise rep5 |
| Sample type |
SRA |
| |
|
| Source name |
Liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
tissue: Liver
exercise during weight loss and maintenance: Treadmill exercise
diet after wlm: ad libitum
time point: First day of weight regain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Livers were removed after 24 hours of refeeding and immediately flash frozen. RNA was isolated using RNeasy kit from Qiagen according to instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
relx25
processed data file: 2019_8_9__Processed_liver_RNAseq_FPKM.xlsx
|
| Data processing |
Trimmomatic to remove sequencing adaptors.
gSNAP for sequence alignments.
Cufflinks to generate FPKM counts.
Genome_build: Rattus_norvegicus.Rnor_5.0.74
Supplementary_files_format_and_content: 2019_8_9__Processed_liver_RNAseq_FPKM.xlsx: Excel file includes FPKM counts.
|
| |
|
| Submission date |
Aug 11, 2019 |
| Last update date |
May 31, 2022 |
| Contact name |
Michael C. Rudolph |
| E-mail(s) |
Michael-rudolph@ouhsc.edu
|
| Organization name |
OUHSC
|
| Department |
Physiology
|
| Lab |
Rudolph Lab
|
| Street address |
975 NE 10th St. BRC North rm 364
|
| City |
Oklahoma City |
| State/province |
OK |
| ZIP/Postal code |
73104 |
| Country |
USA |
| |
|
| Platform ID |
GPL25947 |
| Series (1) |
| GSE135700 |
Regular exercise potentiates energetically expensive hepatic de novo lipogenesis during early weight regain |
|
| Relations |
| BioSample |
SAMN12558642 |
| SRA |
SRX6697198 |
