|
| Status |
Public on Nov 29, 2019 |
| Title |
P3_TGF-β1_0h |
| Sample type |
RNA |
| |
|
| Source name |
dermal fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: dermal fibroblasts
disease state: patient with chronic mucocutaneous candidiasis and connective tissue disorder
mutation: MAPK8 c.311+1G>A
treatment: untreated
|
| Treatment protocol |
Cells were left untreated or treated for 2, 6, or 24 hours with TGF-β1 (10 ng/ml)
|
| Growth protocol |
Dermal fibroblasts were cultured in DMEM supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the RNeasy Plus Micro Kit from QIAGEN
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Data processing |
The data were analyzed with R with RMA normalization
|
| |
|
| Submission date |
Sep 09, 2019 |
| Last update date |
Nov 30, 2019 |
| Contact name |
Tanwir Habib |
| E-mail(s) |
thabib@sidra.org
|
| Organization name |
Sidra Medicine
|
| Street address |
Al Luqta Street
|
| City |
Doha |
| ZIP/Postal code |
26999 |
| Country |
Qatar |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE137107 |
Human JNK1 haploinsufficiency: a novel genetic etiology of chronic mucocutaneous candidiasis and connective tissue disorder |
| GSE137110 |
Chronic mucocutaneous candidiasis and connective tissue disorder in humans with impaired JNK1-dependent responses to IL-17A/F and TGF-? |
|
