|
| Status |
Public on Jul 09, 2021 |
| Title |
control_diet_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
liver_control chow
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
genotype/variation: wild type
Sex: male
age: 30 weeks
diet: control chow
tissue: liver
|
| Treatment protocol |
To promote diet-induced obesity (DIO), mice were ad-libitum fed with 58% high-fat diet (HFD) (Research Diets, D12331) for 22 weeks. Control mice were kept with normal chow. Mice were fasted for 4 hours and then sacrificed by cervical dislocation for organ withdrawal. Livers were removed immediately, flash frozen in liquid nitrogen and stored at -80°C until further analysis.
|
| Growth protocol |
All experiments were performed in adult male C57BL/6J mice purchased from Janvier Labs (Saint-Berthevin, Cedex, France). Mice were maintained on a 12-h light-dark cycle with free access to water and standard chow diet (Altromin, #1314) or 58% high fat diet (HFD) (Research Diets, D12331).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from liver tissue employing a commercially available kit (NucleoSpin RNA, #740955, Macherey-Nagel, Düren, Germany)
|
| Label |
biotin
|
| Label protocol |
Total RNA (150 ng) was amplified using the WT PLUS Reagent Kit (Thermo Fisher Scientific Inc., Waltham, USA).
|
| |
|
| Hybridization protocol |
According to the Thermo Fisher Scientific expression protocol (HWS kit)
|
| Scan protocol |
According to the Thermo Fisher Scientific expression protocol, Scanner 3000 7G
|
| Description |
Gene expression data from liver of mice treated with control diet for 22 weeks, biological replicate 3
|
| Data processing |
SST-RMA gene-level probeset summary with standard settings of the Thermo Fisher Scientific Expression Console software (v.1.4.1.46). The provided SST-RMA data was obtained by including samples from an additional dataset (GSE137924)
|
| |
|
| Submission date |
Sep 24, 2019 |
| Last update date |
Jul 09, 2021 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL24242 |
| Series (1) |
| GSE137923 |
Correlation guided Network Integration (CoNI) reveals novel genes affecting hepatic metabolism |
|
