|
| Status |
Public on Feb 28, 2005 |
| Title |
WT_Gal_N2_0.04_Generations_Rep_1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
WT_Gal_N2_0.04_Generations_Rep_1
|
| Organism |
Saccharomyces cerevisiae |
| Extracted molecule |
total RNA |
| |
|
| Channel 2 |
| Source name |
WT_Gal_N2_Reference
|
| Organism |
Saccharomyces cerevisiae |
| Extracted molecule |
total RNA |
| |
|
| |
| Description |
Sparged, fermentor clutures of a yeast strain (JM43) were aerobically grown on galactose medium (SSG-TEA). At time zero (generation 0) the saprge gas was switched from air to O2-free N2 and samples were harvested at 0.04 generations of anaerobic growth. 30 µg of total RNA was reverse transcribed (Cy3) and hybridized against a reference (Cy5) under the same gas condition, which consisted of a pool of equal masses of RNA from each time point sampled. The data are background-corrected and normalized.
|
| |
|
| Submission date |
Feb 04, 2005 |
| Last update date |
Aug 22, 2008 |
| Contact name |
Kurt E Kwast |
| E-mail(s) |
kwast@uiuc.edu
|
| Phone |
217-244-3122
|
| Organization name |
University of Illinois at Urbana-Champaign
|
| Department |
Molecular & Integrative Physiology
|
| Lab |
Kwast Lab
|
| Street address |
407 S. Goodwin Ave.
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platform ID |
GPL1535 |
| Series (4)
|
| GSE1879 |
aerobic_to_anaerobic_shift |
| GSE2246 |
JM43_Galactose_Air_N2_Air_Shift |
| GSE3705 |
JM43 cells grown aerobically in galactose and treated with Antimycin A |
| GSE3706 |
JM43_Galactose_N2_AntimycinA |
|
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