|
| Status |
Public on Mar 04, 2010 |
| Title |
HCL3 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood cd19+ tumor cells
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: HCL (Hairy Cell Leukemia)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene chip Scanner 3000 7G.
|
| Description |
no additional information
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Jun 05, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Pedro Jares |
| E-mail(s) |
pjares@clinic.ub.es
|
| Organization name |
IDIBAPS
|
| Department |
Genomics Unit
|
| Street address |
c/ Villarroel 170
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE16455 |
Indolent MCL identified by genomic and gene expression profiling |
|
| Relations |
| Reanalyzed by |
GSE119087 |
