|
| Status |
Public on Oct 09, 2020 |
| Title |
tgs1KO-2L-Rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Second instar larvae
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: tgs12-3
tissue: Whole larvae
age: Second instar larvae
|
| Growth protocol |
All of the fly stocks were cultured at 25℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For RNA sequencing, total RNA was extract from about 100 testes or 80 second instar larvae using TRIzol reagent.
mRNA was enriched by using the NEBNext Poly(A) mRNA Magnetic Isolation Module , then an RNA-Seq library were prepared with the NEBNext Ultra RNA Library Prep Kit for Illumina, the libraries were sequenced with Illumina HiSeq X Ten.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
CRISPR/Cas9 mediated knock out of tgs1
Larvae GeneFPKM.txt
|
| Data processing |
data processing step
Supplementary_files_format_and_content: FPKM
|
| |
|
| Submission date |
Jan 12, 2020 |
| Last update date |
Oct 09, 2020 |
| Contact name |
Lin Cheng |
| E-mail(s) |
chengl28@mail2.sysu.edu.cn
|
| Organization name |
Sun Yat-sen University
|
| Street address |
No.132, East Outer Ring Road, Guangzhou University City
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510006 |
| Country |
China |
| |
|
| Platform ID |
GPL23702 |
| Series (1) |
| GSE143488 |
Loss of the RNA trimethylguanosine cap is compatible with nuclear accumulation of spliceosomal snRNAs but not pre-mRNA splicing or snRNA processing during animal development |
|
| Relations |
| BioSample |
SAMN13830745 |
| SRA |
SRX7544107 |
