|
Status |
Public on Mar 21, 2012 |
Title |
C2C12_1.5hr_differentiation_rep3 |
Sample type |
RNA |
|
|
Source name |
C2C12 myoblasts, differentiating, 1.5h
|
Organism |
Mus musculus |
Characteristics |
cell line: C2C12 time point: 1.5hr_differentiation replicate: 3
|
Treatment protocol |
Cells were differentiated by changing the medium to DMEM + 2% horse serum.
|
Growth protocol |
C2C12 myoblasts were cultured in DMEM + 20% FBS before inducing differentiation by changing to DMEM + 2% horse serum.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
cRNA amplification and labeling with biotin were performed using Illlumina TotalPrep RNA amplification kit, manufactured by Ambion, Inc. (Austin, TX) according to the manufacturer’s protocol.
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|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol using the Illumina BeadArray Reader confocal scanner
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Description |
Gene expression data from differentiating C2C12 myoblasts grown in 2% horse serum 1562232003_C
|
Data processing |
The data was processed using the FlexArray package and normalized using LUMI
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|
|
Submission date |
Jul 10, 2009 |
Last update date |
Mar 21, 2012 |
Contact name |
Saravanan Sundararajan |
Organization name |
McGill University
|
Department |
Human Genetics
|
Lab |
Rob Sladek
|
Street address |
740, Dr Penfield Ave
|
City |
Montreal |
State/province |
Quebec |
ZIP/Postal code |
H3A 1A4 |
Country |
Canada |
|
|
Platform ID |
GPL6105 |
Series (2) |
GSE17038 |
Expression Profiling of Early Myogenesis - Illumina Dataset |
GSE17039 |
Expression Profiling of Early Myogenesis |
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