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Status |
Public on Jan 15, 2010 |
Title |
Constitutively active STAT3 cell line 2, replicate 1 |
Sample type |
RNA |
|
|
Source name |
Tumor-derived cell line 2, constitutively active STAT3
|
Organism |
Mus musculus |
Characteristics |
genotype: Constitutively active STAT3
|
Treatment protocol |
none
|
Growth protocol |
Cells were cultured in RPMI 1360 supplemented with FCS 20%, 2 mM Glutamax, 100 U/ml Pennicillin, 100 ug/ml Streptomycin and mainteined at 37°C in a 5% CO2 atmosphere
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with Trizol (Invitrogen) followed by purification on a Qiagen RNAeasy midi column
|
Label |
biotin
|
Label protocol |
RNA amplification and labelling were performed using the Ambion Illumina TotalPrep RNA Amplification Kit
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|
|
Hybridization protocol |
For the hybridization step the samples are applied to the arrays and assembled into the BeadChip Hyb Chamber. The BeadChip is hybridized at 58°C overnight, and then washed the following day.
|
Scan protocol |
Scanning was performed using Illumina Beadarray scanner
|
Description |
none
|
Data processing |
Raw expression data were obtained with BeadStudio, version 3.1.7. Normalization was performed with the lumi package of Bioconductor and default parameters
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|
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Submission date |
Jul 17, 2009 |
Last update date |
Jan 12, 2010 |
Contact name |
Paolo Provero |
E-mail(s) |
paolo.provero@unito.it
|
Organization name |
University of Turin
|
Department |
Molecular Biotechnology and Health Sciences
|
Street address |
Via Nizza 52
|
City |
Torino |
ZIP/Postal code |
I-10100 |
Country |
Italy |
|
|
Platform ID |
GPL6105 |
Series (1) |
GSE17182 |
Gene expression profiling of tumor cell lines with constitutively active STAT3 |
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