|
| Status |
Public on Jan 04, 2022 |
| Title |
S2 |
| Sample type |
SRA |
| |
|
| Source name |
Liquid culture
|
| Organism |
Candida albicans |
| Characteristics |
spike-in: 10 microM oestrogen in ethanol
strain: SC5314
replicate: 2
|
| Growth protocol |
Cells were grown in YPD supplemented or not with 10 µM oestrogen at 37c, 200 rpm.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy kit (Qiagen)
RNA libraries were produced using the Biomek FxP (Beckman Coulter A31842) with NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolab E7420L) and NEBnext Multiplex Oligos for Illumina Dual Index Primers (New England Biolabs E7600S)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
Oestrogen 2
|
| Data processing |
Trim reads, CLC Genomics Workbench 11.0.1
Reads alignement, CLC Genomics Workbench 11.0.1
Genome_build: Candida genome database : http://www.candidagenome.org/ Sequences available here: http://www.candidagenome.org/download/sequence/C_albicans_SC5314/Assembly21/current/, C_albicans_SC5314_version_A21-s02-m09-r10
Supplementary_files_format_and_content: TPM
|
| |
|
| Submission date |
Feb 13, 2020 |
| Last update date |
Jan 04, 2022 |
| Contact name |
Rebecca Anne Hall |
| E-mail(s) |
r.a.hall@kent.ac.uk
|
| Organization name |
University of Kent
|
| Department |
School of Biosciences
|
| Street address |
Giles Lane
|
| City |
Canterbury |
| ZIP/Postal code |
CT2 7NJ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL24129 |
| Series (1) |
| GSE145240 |
Oestrogen promotes innate immune invasion of Candida albicans through inactivation of complement |
|
| Relations |
| BioSample |
SAMN14093888 |
| SRA |
SRX7717673 |
