|
| Status |
Public on Jul 29, 2009 |
| Title |
Muscle diet-sensitive subject 3 |
| Sample type |
RNA |
| |
|
| Source name |
Rectus femoris muscle
|
| Organism |
Homo sapiens |
| Characteristics |
phenotype: obese
gender: Female
tissue: Rectus femoris muscle
responsiveness: diet-sensitive
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared following 2 rounds of low-template amplification according to protocol from Affymetrix (GeneChip Eukaryotic Small Sample Target Labeling Assay Version II Technical Note. Santa Clara, CA.), starting from 400 ng of total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U-133A array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
n/a
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Jul 28, 2009 |
| Last update date |
Jul 28, 2009 |
| Contact name |
Joan Stuart |
| E-mail(s) |
joan.s.stuart@gsk.com
|
| Organization name |
GSK
|
| Street address |
5 Moore Drive
|
| City |
RTP |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE17371 |
Gene Expression Analysis in Skeletal Muscle from Obese, Diet-Sensitive and Diet-Resistant Women |
|
