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| Status |
Public on Jun 01, 2020 |
| Title |
ControlSYN3_1 |
| Sample type |
SRA |
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| Source name |
Fungus
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| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: wild-type
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| Extracted molecule |
total RNA |
| Extraction protocol |
Strains were quickly frozen on dry ice, ground to powder with liquid nitrogen, and RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using oligo -dT beads
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
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| Description |
ControlSYN3_1
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| Data processing |
Basecalls performed using BGISEQ-500
SOAPnuke was used to get the "clean reads".
Mapping clean reads to ControlSYN3_1 whole genome using BWA.
Bowtie2 was used to get the mapping ratio.
Fragments Per Kilobase Million (FPKM) was calculated based on the RSEM.
Genome_build: R64
Supplementary_files_format_and_content: tab-delimited text files include fpkm values for each Sample ...
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| Submission date |
Mar 16, 2020 |
| Last update date |
Jun 01, 2020 |
| Contact name |
peixia wang |
| E-mail(s) |
pxwang@tju.edu.cn
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| Organization name |
天津大学
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| Street address |
天津市津南区海河教育园区雅观路135号天津大学北洋园校区54教203
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| City |
天津市 |
| State/province |
505 |
| ZIP/Postal code |
300350 |
| Country |
China |
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| Platform ID |
GPL25927 |
| Series (1) |
| GSE147008 |
Transcription Analysis of Clustered Essential Genes |
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| Relations |
| BioSample |
SAMN14381355 |
| SRA |
SRX7914812 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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