|
Status |
Public on Sep 05, 2009 |
Title |
Liver Hfeko rep1 |
Sample type |
RNA |
|
|
Source name |
Mouse liver, Hfe knockout
|
Organism |
Mus musculus |
Characteristics |
gender: male strain: C57BL/6 tissue: liver age: 10 weeks
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with RNeasy RNA isolation kit including RNase-free Dnase treatment (Qiagen) according to the manufacturer's protocol. Concentration and purity were measured with Nanodrop.
|
Label |
biotin
|
Label protocol |
Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion) was used following the manufacturer's protocol. Input amount was 300ng total RNA and duration of labeling reaction incubation was 14 hrs.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol (18h at 55 degrees) was used).
|
Scan protocol |
Standard scan factor in Illumina BeadArray reader was used.
|
Description |
no additional information
|
Data processing |
Array data were normalized with Chipster version 1.1.1 using the quantile normalization method.
|
|
|
Submission date |
Sep 04, 2009 |
Last update date |
Sep 04, 2009 |
Contact name |
Alejandra Rodriguez-Martinez |
E-mail(s) |
alejandra.rodriguez.martinez@uta.fi
|
Organization name |
University of Tampere
|
Department |
Institute of Biomedical Technology
|
Street address |
Biokatu 6
|
City |
Tampere |
ZIP/Postal code |
33520 |
Country |
Finland |
|
|
Platform ID |
GPL6105 |
Series (1) |
GSE17969 |
Effects of Hfe-/- and dietary iron overload on gene expression in the liver and duodenum of mice |
|