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Sample GSM45214

Query DataSets for GSM45214

Status

Public on May 15, 2005

Title

treatment=AP time=3 (hrs) AP1-3hr

Sample type

RNA

Source name

Human polymorphonuclear (PMN) leukocytes

Organism

Homo sapiens

Extracted molecule

total RNA

Description

Polymorphonuclear (PMN) leukocytes were obtained from healthy individuals. "time" indicates time at which transcript levels were analyzed (hours). "treatment" indicates whether the polymorphonuclear leukocytes were untreated (ctrl) or treated with Anaplasma phagocytophilum (AP), Staphylococcus aureus (SA), HL60 cell lysate (uninf), heat-killed Anaplasma phagocytophilum (hkAP), or heat-killed HL60 cell lysate (hklys).
Sample_descripton: Sample_PMN_treatment = AP
Sample_descripton: Sample_time = 3
Sample_descripton: Affymetrix MAS version = MAS5
Sample_descripton: GeneSpring (version 6.0)normalization protocol:
Sample_descripton: 1. Data Transformation: Set measurements less than 0.0 to 0.0
Sample_descripton: 2. Per Chip: Normalize to a constant value of 150.0 (MAS scaling factor)
Sample_descripton: 3. Per Gene: Normalize to median
Keywords = NEUTROPHIL RESPONSE TO ANAPLASMA PHAGOCYTOPHILUM

Submission date

Mar 14, 2005

Last update date

May 29, 2005

Contact name

Frank R. DeLeo

E-mail(s)

fdeleo@niaid.nih.gov

Phone

406-363-9448

Fax

406-363-9394

Organization name

National Institute of Allergy and Infectious Diseases

Department

Laboratory of Human Bacterial Pathogenesis

Lab

Rocky Mountain Laboratories

Street address

903 South 4th Street

City

Hamilton

State/province

ZIP/Postal code

59840

Country

USA

Platform ID

GPL96

Series (1)

GSE2405

NIH/NIAID Neutrophil Response to A. phagocytophilum

Data table header descriptions
ID_REF	probeset
VALUE	"signal" a measure of the abundance of a transcript from MAS5
ABS_CALL	the call in an absolute analysis (MAS5) that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
RAW	normalized value computed using GeneSpring version 6.0 software

Data table
ID_REF	VALUE	ABS_CALL	RAW
1007_s_at	0.997	M	121.8
1053_at	0.693	A	16.9
117_at	1.396	P	209.1
121_at	0.896	P	419.3
1255_g_at	0.242	A	5.8
1294_at	1.83	P	171.8
1316_at	1.072	P	86.7
1320_at	4.017	A	23.7
1405_i_at	1.97	P	349.4
1431_at	0.912	A	20.6
1438_at	1.544	A	23
1487_at	0.998	P	289.9
1494_f_at	1.225	P	148.6
1598_g_at	0.641	A	127.3
160020_at	0.948	A	191.4
1729_at	1.096	P	114.3
1773_at	0.984	A	25.1
177_at	1.483	P	66.9
179_at	0.766	A	232.1
1861_at	1.014	A	35.6

Total number of rows: 22283

Table truncated, full table size 514 Kbytes.

Supplementary data files not provided