|
| Status |
Public on May 17, 2021 |
| Title |
Fat body-ND-5# |
| Sample type |
SRA |
| |
|
| Source name |
Fat body-ND
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Oregon-R
genotype/variation: WT
age: 5days
treatment: normal food diet
tissue: Fat body
|
| Treatment protocol |
The brains and fat body were harvest directly.
|
| Growth protocol |
Flies were cultured in normal food or high sugar diet for 5d.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was harvested using the RNeasy Mini kit, subjected to poly(A) mRNA isolation, cDNA synthesization, library preparation (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB, NEB)
RNA libraries were prepared for sequencing using standard NEB protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
raw reads were mapped on dm3 genome by TopHat using the default settings. We counted the uniquely mapped reads in the exon regions of genes and calculate the RPKM values of the genes based on these reads.
Genome_build: Drosophila melanogaster genome (r3)
Supplementary_files_format_and_content: RPKM was used to quantify gene expression for each sample
|
| |
|
| Submission date |
May 14, 2020 |
| Last update date |
May 18, 2021 |
| Contact name |
Danping Chen |
| E-mail(s) |
11707005@zju.edu.cn
|
| Organization name |
Zhejiang Univerisity
|
| Lab |
Wang Lab
|
| Street address |
866 Yuhangtang Rd
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platform ID |
GPL23702 |
| Series (1) |
| GSE150565 |
A diet-induced type 2 diabetes model in Drosophila |
|
| Relations |
| BioSample |
SAMN14924098 |
| SRA |
SRX8341625 |
