|
| Status |
Public on Feb 20, 2010 |
| Title |
Mtf2_RNAi_3_2days |
| Sample type |
RNA |
| |
|
| Source name |
Mtf2_RNAi_3, 2days
|
| Organism |
Mus musculus |
| Characteristics |
cell line: E14 mESC
|
| Treatment protocol |
Plasmids were transfected into the E14 mESC cell lines by lipofectamine 2000 and selected by Puromycin (1 mg/ml) after 1 day of transfection. The sample of 2 days maintained in puromycin was harvested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted by Trizol Reagent(Invitrogen 15596-018)
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 1 ug RNA using the Quick-Amp One-Color (Agilent) according to the manufactruers instructions. Total amount of cRNA and dye incorporation was measured using a nanodrop instrument.
|
| |
|
| Hybridization protocol |
825 ng of labeled RNA was fragmented and hybridized on the slide in a rotating hybridzation oven at 60C overnight. Slides were washed according to the manufactureres protocols using Agilent wash buffers.
|
| Scan protocol |
Slides were scanned in an Axon 4000B scanner using GenePix 6 software to generate gpr files.
|
| Description |
gene expression of cells transfected with plasmid pSuper-Mtf2-RNAi-3 and selected by Puromycin(1mg/ml) for 2 days
|
| Data processing |
Data normalized with dChip.
|
| |
|
| Submission date |
Sep 29, 2009 |
| Last update date |
Feb 20, 2010 |
| Contact name |
Dan Xie |
| E-mail(s) |
danxie2@uiuc.edu
|
| Organization name |
University of Illinois at Urbana and Champaign
|
| Department |
Bioengineering
|
| Street address |
3215 Digital Computer Lab, MC-278, 1304 W. Springfield Ave
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE18319 |
Gene expression signatures for mESC treated by RNAi strategy on down regulation of gene Mtf2 |
|
