|
| Status |
Public on Sep 20, 2010 |
| Title |
CF_lubiprostone |
| Sample type |
RNA |
| |
|
| Source name |
Entire small intestine, CF, lubiprostone treated
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: small intestine
genotype: cftr-tm1Unc (cftr null)
treatment: lubiprostone
|
| Treatment protocol |
Mice were gavaged daily with 100 ul Peptamen including 10 ug/kg body weight lubiprostone for two weeks, as indicated.
|
| Growth protocol |
All mice were fed from weaning to sacrifice with a liquid diet (Peptamen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The entire small intestine was resected into ice cold PBS; the mesentery was trimmed and discarded; the tissue was homogenized in 8 ml Trizol and extracted to isolate total RNA.
|
| Label |
biotin
|
| Label protocol |
The standard labeling protocol was used.
|
| |
|
| Hybridization protocol |
GeneChip was prehybridized with Hybridization buffer at 45C 10min.
Denatured Hybridization cocktail was applied to GeneChip.
GeneChip was hybridized 16 hr at 45C in GeneChip Hybridization Oven 640.
Washing and Staining: Automated process using the GeneChip Fluidics Station 400 with the Standard Array Format.
|
| Scan protocol |
Automated process conducted using the Agilent GeneArray Scanner.
Pixel value = 3um
Wavelength = 570nm
|
| Description |
CFIL_070908
|
| Data processing |
Primary analysis was by GCOS with 'Scaling - All Probe Sets: Target Signal = 500'; 'Normalization – User Defined: Scale Factor = 1' using MAS 5.0.
|
| |
|
| Submission date |
Sep 29, 2009 |
| Last update date |
Sep 20, 2010 |
| Contact name |
Robert C De Lisle |
| E-mail(s) |
rdelisle@kumc.edu
|
| Phone |
913-588-2742
|
| Organization name |
Univ Kansas Med Ctr
|
| Department |
Anatomy & Cell Biology
|
| Street address |
3901 Rainbow Blvd
|
| City |
Kansas City |
| State/province |
KS |
| ZIP/Postal code |
66160 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE18327 |
Lubiprostone effects on small intestinal gene expression in wild type and Cftr-null mice |
|
