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Status |
Public on Oct 01, 2010 |
Title |
Spinal cord ependymal cells rep 2 |
Sample type |
RNA |
|
|
Source name |
Spinal cord ependymal cells, FACS isolated, CD133+/CD24+/CD45neg/CD34neg
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6 developmental stage: adult cell source: FACS sorted from tissue facs phenotype: D133+/CD24+/CD45neg/CD34neg
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted and purified with QIAgen's RNeasy Micro Kit including DNase I treatment
|
Label |
biotin
|
Label protocol |
RNA amplification and biotin-labeling were achieved with the TargetAmp 2-Round Biotin-aRNA Amplification Kit (Epicentre)
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
SC_02
|
Data processing |
Raw data was background-corrected and summarized using BeadStudio software (Illumina) and further processed with the Bioconductor/Lumi package; data were normalized applying quantile normalization and variance stabilization (function vst).
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|
|
Submission date |
Oct 13, 2009 |
Last update date |
Oct 13, 2009 |
Contact name |
Ulrike A. Nuber |
Organization name |
Lund University
|
Street address |
Klinikgatan 26
|
City |
Lund |
ZIP/Postal code |
SE-22184 |
Country |
Sweden |
|
|
Platform ID |
GPL6105 |
Series (1) |
GSE18528 |
Comparative analysis of ependymal cells from the lateral ventricular wall and the spinal cord of adult mice |
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