|
| Status |
Public on Oct 01, 2021 |
| Title |
DE160/DE160-D35-rep2 |
| Sample type |
SRA |
| |
|
| Source name |
HESC-derived cardiomyocytes
|
| Organism |
Homo sapiens |
| Characteristics |
disese state: day35 (D35) post cardiac differentiation
genotype: cTnT-DE160 mutation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total mRNAs were isolated from normal and mutant H7-derived cardiomyocytes on day 14 and day 35 post differentiation using TRIZOL (Invitrogen) according to the directions. Poly(A) RNA was purified from total RNA using the MicroPoly(A)TM kit (Life Technologies).
1 ug of total RNA was enriched for the Poly-A mRNA and reversed to cDNA, which was end-repaired, 3' ends adenylated, and barcoded with multiplex adapters.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
sample_29
|
| Data processing |
RNA sequencing reads were quality filtered and processed using the pseudoalignment-based Kallisto algorithm with GRCh37 comprehensive transcript set as reference transcriptome.
none provided by the submitter
Genome_build: GRCh37
Supplementary_files_format_and_content: Tab-delimited text files include gene counts for every gene and every sample.
|
| |
|
| Submission date |
Jul 09, 2020 |
| Last update date |
Oct 01, 2021 |
| Contact name |
Bin Li |
| E-mail(s) |
14111010069@fudan.edu.cn
|
| Organization name |
Fudan University
|
| Department |
Department of Physiology and Pathophysiology
|
| Street address |
Dong'an Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE154097 |
Isogenic human pluripotent stem cell disease models reveal actin binding Rho activating protein deficiency underlies the cardiac troponin T DK210 mutation-induced familial dilated cardiomyopathy II |
|
| Relations |
| BioSample |
SAMN15493289 |
| SRA |
SRX8699776 |
