|
Status |
Public on Oct 31, 2009 |
Title |
donor 1, H5N1 infected macrophages at 6h |
Sample type |
RNA |
|
|
Source name |
macrophages from donor 1 infected with H5N1 at 6h post-infection time
|
Organism |
Homo sapiens |
Characteristics |
individual: donor 1 infection: H5N1 time point: 6h post-infection time cell type: monocyte-derived macrophages
|
Treatment protocol |
Differentiated macrophages were infected with H1N1, H5N1 or mock infection at a multiplicity of infection (MOI) of two. Total RNA was extracted from cells after 1, 3, and 6h post-infection
|
Growth protocol |
Peripheral-blood leucocytes were separated from buffy coats of healthy donors by Ficoll-Paque density gradient centrifugation and purified by adherence. The cells were allowed to differentaite for 14 days in RPMI 1640 supplemented with 5% heat-inactivated autologous plasma.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated by RNEasy Mini kit (Qiagne) according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Total RNA was labeled employing the Whole Transcript (WT) Sense Target Labeling Assay according to the manufacturer’s instructions (Affymetrix).
|
|
|
Hybridization protocol |
The samples were hybridized to Human Gene 1.0 ST microarrays according to standardized protocols from Affymetrix.
|
Scan protocol |
The samples were scanned on a GeneChip Scanner 3000 7G (Affymetrix).
|
Description |
Gene expression data of influenza A H5N1 infected macrophages at 6 h post-infection time
|
Data processing |
The microarray data were preprocessed with Affymetrix Expression Console software using Affymetrix default Robust Multichip Analysis (RMA) sketch algorithm workflow.
|
|
|
Submission date |
Oct 30, 2009 |
Last update date |
Sep 01, 2016 |
Contact name |
SMY Lee |
Organization name |
The University of Hong Kong
|
Street address |
21 Sassoon Road
|
City |
Hong Kong |
ZIP/Postal code |
HKG |
Country |
Hong Kong |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE18816 |
Expression data of influenza A infected human macrophages |
|
Relations |
Reanalyzed by |
GSE86357 |