|
| Status |
Public on Nov 04, 2009 |
| Title |
Dmel_loq_sRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
4-6 day old adult flies
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: loquacious mutant
rna: 18-35 small RNAs
gender: female
|
| Growth protocol |
Standard fly husbandry
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Library constructed using the SOLiD Small RNA expression kit, directional cloning of small RNAs based on a 5' Phosphate group
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
AB SOLiD System 2.0 |
| |
|
| Description |
small RNA discovery and profiling
|
| Data processing |
Reads were aligned to the D. melanogaster (build: dm3) and C virus genomes allowing 6 colourspace matches, with up to 3 mismatches in the first 18 colours. Sequencing adaptors were removed using the method of the ABI small RNA Analysis Toolkit.
|
| |
|
| Submission date |
Nov 03, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Trevis M Alleyne |
| E-mail(s) |
t-alleyne@northwestern.edu
|
| Phone |
312-503-9257
|
| Fax |
312-503-3210
|
| Organization name |
Northwestern University
|
| Department |
Feinberg School of Medicine
|
| Lab |
Center for Genetic Medicine
|
| Street address |
303 E. Superior Street
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL9521 |
| Series (1) |
| GSE18871 |
The small RNA profile in wildtype, R2D2 and loquacious mutant Drosophila |
|
| Relations |
| SRA |
SRX016018 |
| BioSample |
SAMN00008205 |
