|
Status |
Public on Nov 04, 2009 |
Title |
Dmel_loq_sRNAseq |
Sample type |
SRA |
|
|
Source name |
4-6 day old adult flies
|
Organism |
Drosophila melanogaster |
Characteristics |
genotype: loquacious mutant rna: 18-35 small RNAs gender: female
|
Growth protocol |
Standard fly husbandry
|
Extracted molecule |
total RNA |
Extraction protocol |
Library constructed using the SOLiD Small RNA expression kit, directional cloning of small RNAs based on a 5' Phosphate group
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
AB SOLiD System 2.0 |
|
|
Description |
small RNA discovery and profiling
|
Data processing |
Reads were aligned to the D. melanogaster (build: dm3) and C virus genomes allowing 6 colourspace matches, with up to 3 mismatches in the first 18 colours. Sequencing adaptors were removed using the method of the ABI small RNA Analysis Toolkit.
|
|
|
Submission date |
Nov 03, 2009 |
Last update date |
May 15, 2019 |
Contact name |
Trevis M Alleyne |
E-mail(s) |
t-alleyne@northwestern.edu
|
Phone |
312-503-9257
|
Fax |
312-503-3210
|
Organization name |
Northwestern University
|
Department |
Feinberg School of Medicine
|
Lab |
Center for Genetic Medicine
|
Street address |
303 E. Superior Street
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60611 |
Country |
USA |
|
|
Platform ID |
GPL9521 |
Series (1) |
GSE18871 |
The small RNA profile in wildtype, R2D2 and loquacious mutant Drosophila |
|
Relations |
SRA |
SRX016018 |
BioSample |
SAMN00008205 |